Evaluation of Antagonistic Ability of a Local Isolate of Trichoderma harzianum Against Rhizoctonia solani
DOI:
https://doi.org/10.63359/z61dqa57Keywords:
Trichoderma harzianum،, Rhizoctonia solani, Antagonistic., FungiAbstract
In this study, a local isolate of the antagonistic fungus Trichoderma harzianum was used to evaluate its antagonistic capacity against the pathogenic fungus Rhizoctonia solani, such as competition, antagonism, and mycoparasitism, and to use it as a seed coat or in soil injection to study its effect on seed germination and to resist seed rot and seedling damping-off disease caused by R. solani. The results showed that T. harzianum significantly affects the growth of the pathogen, directly or indirectly. Microscopic examination showed that the antagonistic fungal hyphae grew parallel to the pathogenic hyphae, coiling around them in a spiral fashion, and forming protrusions that punctured the mycelium of the pathogenic fungus. Treatment with the antagonistic fungus T. harzianum, whether as a seed coat or injected into the soil before planting, improved the germination rate by 64.7%, significantly reduced seed rot to 35.3%, and increased the percentage of healthy seedlings to 60% in the presence of the pathogenic fungus R. solani.
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